Molecular Interaction between Lipoteichoic Acids and Lactobacillus delbrueckii Phages Depends on D-Alanyl and -Glucose Substitution of Poly(Glycerophosphate) Backbones
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Molecular Interaction between Lipoteichoic Acids and Lactobacillus delbrueckii Phages Depends on D-Alanyl and -Glucose Substitution of Poly(Glycerophosphate) Backbones Liisa Räisänen,* Christian Draing, Markus Pfitzenmaier, Karin Schubert, Tiina Jaakonsaari, Sonja von Aulock, Thomas Hartung, and Tapani Alatossava Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland; Department of Biochemical Pharmacology, University of Konstanz, D-78457 Konstanz, Germany; Institute for Organic Chemistry, Philipps-Universität Marburg, D-35043 Marburg, Germany; Department Biologie I, Bereich Mikrobiologie der Universität München, D-80638 Munich, Germany; Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland; European Centre for the Validation of Alternative Methods, IHCP, JRC, 21020 Ispra, Italy; and Department of Food Technology, University of Helsinki, P.O. Box 66, FIN-00014 Helsinki, Finland
منابع مشابه
Molecular interaction between lipoteichoic acids and Lactobacillus delbrueckii phages depends on D-alanyl and alpha-glucose substitution of poly(glycerophosphate) backbones.
Lipoteichoic acids (LTAs) have been shown to act as bacterial counterparts to the receptor binding proteins of LL-H, LL-H host range mutant LL-H-a21, and JCL1032. Here we have used LTAs purified by hydrophobic interaction chromatography from different phage-resistant and -sensitive strains of Lactobacillus delbrueckii subsp. lactis. Nuclear magnetic resonance analyses revealed variation in the ...
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Lipoteichoic acids (LTAs) were purified from Lactobacillus delbrueckii subsp. lactis ATCC 15808 and its LL-H adsorption-resistant mutant, Ads-5, by hydrophobic interaction chromatography. L. delbrueckii phages (LL-H, the LL-H host range mutant, and JCL1032) were inactivated by these poly(glycerophosphate) type of LTAs in vitro in accordance to their adsorption to intact ATCC 15808 and Ads-5 cells.
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Lipophilic and hydrophilic D-alanyl-lipoteichoic acids are elongated in Lactobacillus casei by the transfer of sn-glycerol 1-phosphate units from phosphatidylglycerol to the poly(glycerophosphate) moiety of the polymer. These sn-glycerol 1-phosphate units are added to the end of the poly(glycerophosphate) which is distal to the glycolipid anchor; 1,2-diglyceride results from this addition. The ...
متن کاملInfluence of Alanine Ester and Glycosyl Substitution on the Lip0 teichoic Acid Carrier Activity
Native lipoteichoid acids from Staphylococcus aureus with D-alanine ester/phosphate molar ratios between 0.23 and 0.73 were tested for lipoteichoic acid carrier activity with poly(ribito1 phosphate) polymerase from Staphylococcus aureus H. Lipoteichoic acid with an alanine/phosphorus ratio greater than 0.7 was inactive. With decreasing substitution, lipoteichoic acid carrier activity appeared a...
متن کاملSynthesis of the lipoteichoic acid of the Streptococcus species DSM 8747.
The lipoteichoic acid (LTA) of the Streptococcus species DSM 8747 consists of a beta-d-galactofuranosyl diacylglycerol moiety (with different acyl groups) that is linked via 6-O to a poly(glycerophosphate) backbone; about 30% of the glycerophosphate moieties carry at 2-O hydrolytically labile d-alanyl residues. As typical LTA for this array of compounds LTA 1a was synthesized. To this end, from...
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تاریخ انتشار 2007